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INEM Tech Core

At INEM, technical platforms enhance research efficiency and cost-effectiveness, providing access to instruments and expertise beyond individual labs' means. Skilled personnel assist researchers, offering insights into instrument capabilities and developing new applications. The technical platforms also provide access to specific instruments with expert references, ensuring optimal use.

Radioactivity facility 

Stefano Fumagalli
Stefano Fumagalli
Nathalie Servel
Nathalie Servel

 

Recombinant proteins facility

Pamela Schnupf
Pamela Schnupf
Emeline Pacreau
Emeline Pacreau

 

Experimental platform In Vivo for autoimmune and metabolic diseases

This platform provides project-based services to researchers working on autoimmune and metabolic diseases. Researchers interested in using the platform can contact Emmanuelle Waeckel-Enée directly. 

In order to ensure efficient management of requests and meet specific criteria, researchers will have to complete a detailed form. This will collect information on the project, including technical, logistical and feasibility aspects, as well as details on current projects and associated deadlines.

Contact

  • Engineer : Emmanuelle Waeckel Enée
  • Scientific referent : Elise Dalmas
Emmanuelle Waeckel Enée
Emmanuelle Waeckel Enée
Elise Dalmas
Elise Dalmas

Service request

Please complete the online form.

Services offered

  • In vivo phenotyping of preclinical models of autoimmune and metabolic diseases

    - Monitoring of Type 1 (NOD) and Type 2 (diet-induced obese) diabetic mice (glycemia, glucose tolerance test…)
    - Induction and scoring of mouse experimental autoimmune encephaomyelitis (EAE) 
    - Adoptive transfer cell models and injections (ip, iv, im, subcutaneous, intrathecal…)

  • Isolation of specific tissues and cell types

    - Tissue sampling (brain, spinal cord, bone marrow, lymph nodes, mammary glands, blood...)
    - Isolation of pancreatic islets
    - Tissue digestion and cell sorting 

  • Follow-up of ex vivo/tissue analyses

    - Cell Culture
    - Molecular biology
    - Immunohistochemistry and imaging
    - Confocal microscopy, spinning disk
    - Flow cytometry

 

Bioinformatics Hub

The Bioinformatics Hub of INEM is a collaborative platform dedicated to omics data analysis and integration. Our mission is to provide a centralized space for data analysis, tool development, and bioinformatics training, fostering dynamic collaboration between biologists and bioinformaticians.

Contact

Lamine Touré
Lamine Touré

 

Type of Analyses Offered

We specialize in a wide range of bioinformatics analyses, including:

  • Single-cell RNA-Seq (scRNA-Seq): Data integration, differential expression analysis, gene regulatory networks (GRN), cell-type annotation, trajectory analysis, and more.
  • Bulk RNA-Seq: Alignment, quantification, co-expression analysis, differential expression, biomarker discovery, GRN construction, etc.
  • Epigenomics: Identification of binding sites, epigenetic profiling, chromatin accessibility analysis, etc.
  • Proteomics: Phosphoproteomics pipelines, differential analysis, post-translational modifications studies, protein-protein interactions (PPI), etc.
  • Artificial Intelligence (AI): Machine learning, statistical analysis (descriptive, exploratory, PCA), and reproducibility of methods from research papers.
  • Multi-omics Integration: Combining and analyzing data from multiple omics layers to gain comprehensive biological insights.
  • Interactive Visualization: Development of interactive dashboards and applications for data exploration, tailored to the needs of research teams.

Consulting Services

We provide expert guidance and support for bioinformatics projects:

  • Guidance on Bioinformatics Tools and Pipelines: Assistance with selecting, implementing, and optimizing workflows for data analysis.
  • Project Support and Consultation: Help with troubleshooting, technical questions, and analytical strategies.

All tools and code developed by the Hub are openly available on GitHub (https://github.com/Institut-Necker-Enfants-Malades).

Our Strategic Objectives

  • Building a Bioinformatics Community at INEM: We organize monthly seminars, training sessions, and discussion groups to enhance bioinformatics skills and promote interdisciplinary collaboration.
  • Providing Centralized Computational Resources: We offer access to source codes, applications, servers, and pipelines, empowering researchers to efficiently analyze and interpret their data.
  • Fostering a Flexible and Collaborative Environment: The Hub operates through a hybrid model, with a dedicated bioinformatician (Lamine Touré) and contributions from bioinformaticians across INEM research teams, enabling seamless interdisciplinary interactions.

For inquiries about our bioinformatics services, collaboration opportunities, or technical support, please don't hesitate to reach out. 

 

SFR Necker - Necker Federative Research Structure

The SFR Necker plays a pivotal role in the development and coordination of technical platforms tailored to the technological requirements of projects on the Necker campus. With a primary focus on optimizing financial, technological, and human resources, SFR Necker aims to create an optimal environment for cutting-edge fundamental and clinical research, collaborations and enhanced visibility.

SFR Necker coordinates 15 technical platforms covering areas such as proteomics, cell imaging, flow cytometry, bioinformatics, among others, offering a diverse range of services. These technical platforms are open to the entire scientific community of the Imagine Institute, the Necker Enfants Malades Institute, as well as the broader regional academic and private scientific community.

For more details about this initiative, please visit the SFR Necker website.